Serial dilution lab purpose

Serial dilution generally refers to selection preformed in the standard growth regimes typically used in the lab. Mass per unit volume is often used to report the concentration of proteins and. Serial dilution helps to choose a dilution which is relevant to our experiment. Upon completion of this lab, the student should be able to proficiently calculate molarities for solutions. The diluted sample is then used as the base solution to make an additional dilution. Serial dilution is a simple yet efficient technique to determine the number of cells or organisms in a concentrated sample. Tube agglutination method is the recommended method of performing the widal test. This exercise is intended to familiarize us with using scientific notation to express big and small number easily and the process of using serial dilutions to make. Many laboratory exercises and experiments require one to make serial dilutions of bacterial cultures or virus.

Often the standard which is given to you in the lab is far to strong for the experiment and it needs to be diluted. Usually the dilution factor at each step is constant, resulting in a geometric progression of the. This video shows creation of a series of solutions of different ph by serial dilution. The progression of calibration standard concentration is always a geometric series. In microbiology, serial dilutions log dilutions are used to decrease a bacterial concentration to a required concentration for a specific test method, or to a concentration which is easier to count when plated to an agar plate i have created this guide to provide a better understanding of dilutions and should be used.

A dilution can be performed not only to lower the concentration of the analyte that is being tested, so that it is in range, but also to help eliminate interferences from other substances that may be present in the sample that can. A serial dilution is the stepwise dilution of a substance in solution. Serial dilution simulation lab the american biology teacher. For the solutions to be usable in the lab for a titration, for instance, they must be accurately diluted to a known, lesser concentration.

Importance of serial dilution in serological tests. Importance of serial dilution in serological testsserial dilution lab purpose rating. The levels are measured by using serial dilutions of sera. Protocol for using serial dilutions in the microbiology lab. To perform a serial dilution, a small amount of a wellmixed solution is transferred into a new container, and additional water or other solvent is added to dilute the original solution. Pdf lab report of microbiology akbar haqi academia. Your microbiology lab instructor taught you the basics of serial dilution and how to use it to determine the number of cells in an original bacterial culture. Repeat the steps until the cells can be observed under the microscope when the diluted sample was observed.

As such, microbiologists have employed serial dilution and various plating. Consider the example of making the first standard at the concentration of the known, the next calibrant would be 19th the concentration of the known. Dilutions can be important when dealing with an unknown substance. Many laboratory exercises and experiments require one to make serial dilutions of bacterial cultures or virus cultures in order to calculate the concentration of. First, take a portion of the sample and does serial dilution on it. A serial dilution is a series of stepwise dilutions, where the dilution factor is held constant at each step. A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration. An easy learning guide dilution is the process of making a solution weaker or less concentrated. The dilution factor chosen for the series of calibration standards is achievable by using serial dilution. The easiest method is to make a series of 1 in 10 dilutions.